A peptide was subjected to degradation from the N-terminus (

A peptide was subjected to degradation from the N-terminus (Edman degradation). The resultant mixtur… Show more A peptide was subjected to degradation from the N-terminus (Edman degradation). The resultant mixture was subjected to MALDI/MS analy- sis. The following ladder of m/z was observed: 977.2, 1064.3, 1151.4, 1222.6, 1378.9, and 1465.9. Assume that z = 1 in all cases, and derive the sequence of the N-terminal region of the peptide. The identical peptide was enzy- matically phosphorylated with protein kinase C. For this peptide, the ladder of m/z was as follows: 977.3, 1064.3, 1231.6, 1302.6, 1458.9, and 1545.9. Explain these results. • Show less

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