… Show more For your next temperature experiment, you grow

… Show more For your next temperature experiment, you grow a subsample until it is in exponential growth phase. This you will usually hear called “log phase” in practice. You transfer that to new culture broth at the appropriate temperature and begin the experiment. You sample at the beginning and after 4 hours. Again you make a serial dilution and spread 0.1 ml from each dilution on your growth plates. The data you collect are below. A. Using these dilution series counts, calculate the most accurate values for the concentration of cells in cfu/ml at each time point and temperature: T0 35 C T4 35 C T0 65 C T4 65 C B. Using these values, calculate n after 4 hours (generations), g after 4 hours (in hours), and the specific growth rate (k) for each temperature. Show your calculations and units of final answer. 35 C 65 C C. Which temperature is more optimal for this strain of bacteria? How would we characterize this bacterium with regards to temperature optimum? • Show less

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