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Dissertation – The effect of artificial electrical stimulation on muscle atrophy in skeletal muscle in rats

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The effect of artificial electrical stimulation on muscle atrophy in skeletal muscle in rats.

Dissertation Discussion chapter: Points that might be included are: (i) evaluation of the significance of the results, highlighting any interesting and/or anomalous findings (including mathematical/statistical interpretation, where appropriate), (ii) review of results in relation to previously published work – where there is a limited amount of work in a particular topic, you will need to range more widely in your use of the literature (poor use of references will lead to a poor mark from your assessors), (iii) a final paragraph assessing whether the aims have been achieved and giving the principal conclusion(s) of the study, and (iv) discussion of ‘errors’ should be minimized – accentuate the positive! Some class notes to clear up the pattern of analysis used: – Compare the denervated (3 day, 2 week, 4 week) to its contralateral control using unpaired t-test, as you have only two variables to compare. Do not use ANOVA here. This will give you a timeline of muscle atrophy. – Use ANOVA to compare the random control to all three other contralaterals (3 day, 2 week, 4 week)- this is multiple comparison, and that is why you use ANOVA here. We are assuming that the random controls are representative of animals of same sex, age and weight to the denervated animals. We would like to see whether the denervation has caused excessive use of the chealthy contralateral side (this will be true if the contralateral at any point is bigger than the random controls). ANOVA may or may not indicate a significant difference. If P>0.05 then there is no difference and the animal was not excessively usinbg the intact contralateral leg, and therefore there is no hypertrophy. If P<0.05 then there is a significant difference somewhere and you need to go and do paired t-tests inbetween the variables. The (Norways)in M&M and Results section are the actual stimulated muscles, which you may conclude from through research if artificial electrical stimulation affects atrophied muscles and if the null hypothesis should be rejected or not.