DNases are used for treatment of cystic fibrosis. A normal D

DNases are used for treatment of cystic fibrosis. A normal DNase catalyzes the hydrolytic cleavage o… Show more DNases are used for treatment of cystic fibrosis. A normal DNase catalyzes the hydrolytic cleavage of phosphodiester bond in one strand of DNA. Scientists, in order to improve efficacy of DNases, use hyperactive DNases which cleave both strands of DNA. Here, electrophoresis is performed of a plasmid without DNase, a plasmid digested by normal DNase and 3 plasmids digested with 3 hyperactive DNases. Plasmid without DNase presents 2 bands, plasmid with DNase one band and that with hyperactive DNase 3 bands. a) Why? b) Which of the 3 hyperactive DNase is more efficient? • Show less

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