I have a cell line (HeLa) that I want to grow in culture and

I have a cell line (HeLa) that I want to grow in culture and then test the efficacy of a drug on the… Show more I have a cell line (HeLa) that I want to grow in culture and then test the efficacy of a drug on the production of a protein identified to be anti-proliferative (IκBα). At this point, I have no idea as the EC50 of the drug. Using your knowledge from the course and from the literature describe in detail: A: How to grow HeLa cells, including media and supplements, temperature, gas requirements. B: How to find the EC50 of the drug in terms of increasing expression of IκBα (use the immunoblot to quantitate the amount of protein) C: How to determine where IκBα is expressed in a cell culture using immunohistochemistry. • Show less

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