Polyphenoloxidase (PPO) is an enzyme that functions as a def

Polyphenoloxidase (PPO) is an enzyme that functions as a defense mechanism in some… Show more I think it is B. Polyphenoloxidase (PPO) is an enzyme that functions as a defense mechanism in some plants. You are interested in producing a large quantity of PPO to engineer into plants that are unable to produce this enzyme. The DNA sequence including the gene of interest (segment bolded below the reverse primer) for this enzyme in potatoes is shown below along with the reverse primer: —> 3’ TGG AAA 5’ 5’ TTT TAT GAG CGG ATT CTC GGG AGC CTA ATA GAT AAC CCC ACC TTT GCC 3’ 3’ AAA ATA CTC GCC TAA GAG CCC TCG GAT TAT CTA TTG GGG TGG AAA CGG 5’ question: Which of the following is the correct forward primer that will allow you to best amplify your area of interest and obtain the target PCR product? (0.5 point) Indicate on the DNA sequence above where the forward primer will bind. (0.5 point) A. 5’ CCGTTT 3’ B. 5’ AAAGGT 3’ C. 3’ AAAAUA 5’ D. 3’ TTTTAT 5’ E. 5’ TATGAG 3’ F. 3’ UUUAUC 5’ • Show less

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